The B cell death function of obinutuzumab-HDEL produced in plant (Nicotiana benthamiana L.) is equivalent to obinutuzumab produced in CHO cells.

The B cell death function of obinutuzumab-HDEL produced in plant (Nicotiana benthamiana L.) is equivalent to obinutuzumab produced in CHO cells.

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Plants have attracted attention as bio-drug production platforms because of their economical and safety benefits.The preliminary efficacy of ZMapp, a cocktail of antibodies produced in N.benthamiana (Nicotiana benthamiana here L.

), suggested plants may serve as a platform for antibody production.However, because the amino acid sequences of the Fab fragment are diverse and differences in post-transcriptional processes between animals and plants remain to be elucidated, it is necessary to confirm functional equivalence of plant-produced antibodies to the original antibody.In this study, Obinutuzumab, a third generation anti-CD20 antibody, was produced in N.

benthamiana leaves (plant-obinutuzumab) and compared to the original antibody produced in glyco-engineered Chinese hamster ovary (CHO) cells (CHO-obinutuzumab).Two forms (with or without an HDEL tag) were generated and antibody yields were compared.The HDEL-tagged form was more highly expressed than the non-HDEL-tagged form which was cleaved in the N-terminus.

To determine the duospiritalis.com equivalence in functions of the Fab region between the two forms, we compared the CD20 binding affinities and direct binding induced cell death of a CD20-positive B cells.Both forms showed similar CD20 binding affinities and direct cell death of B cell.The results suggested that plant-obinutuzumab was equivalent to CHO-obinutuzumab in CD20 binding, cell aggregation, and direct cell death via binding.

Therefore, our findings suggest that Obinutuzumab is a promising biosimilar candidate that can be produced efficiently in plants.